Remarkably, the secreted levels of both interferons (IFN-α and IFN-β) exhibit a gradual increase over the time following HSV-2 infection, with a statistically significant surge observed from 8 h post-infection onwards, with the highest levels of expression observed at the later time points from 24 hpi to 48 hpi and downregulation in their expression after 48 hpi (Figure 2A,B). The gene discussed is IFNB1; the disease is infection.