TLR4 and obesity due to melanocortin 4 receptor deficiency: In addition, n-6 PUFAs exacerbated obesity-related osteoarthritis, whereas n-3 PUFAs were protective against the disease by regulating the TLR4/NF-κB and NOD-like receptor protein 3 (NLRP3)/caspase-1/gasdermin D pathways [131].