In a mouse model of CKD, SIRT6-HMGB1 deacetylation can inhibit the transport of HMGB1 from the nucleus to the cytoplasm and induce Runx2, OPN, and muscle segment homeobox 2 (Msx2) expression, thereby regulating CVC in CKD [62]. Here, RUNX2 is linked to chronic kidney disease.