However, IL-1β and TNF-α expression were reduced in the CFA+solanesol group, with no difference observed in the WT+saline and WT+solanesol groups (Figure 3A–D), suggesting that solanesol ameliorates anxiety-like behaviors by downregulating IL-1β and TNF-α in the ACC and had no influence on IL-1β and TNF-α expression in mice without neuro-inflammation. This evidence concerns the gene TNF and Anxiety.