Subsequently, the biological interaction between these genes and miR-200c-3p was explored by employing a combination of computational and molecular analysis resulting in miR-200c-3p regulated directly PRKG1 gene and indirectly SULF1 and SYDE1 genes, influencing the pathogenesis of achalasia through NO/cGMP/PKG signaling [54]. This evidence concerns the gene PRKG1 and Achalasia.