The same PSA-5′tRF(Glu + Val) combined model was able to discriminate (BPH + PCa I + IIA) from (PCa IIB + IIC + IIIB) samples (AUC: 0.736; p = 0.032; Sn: 43.8%, Sp: 85.7%; PPV: 70%, NPV: 66.7%), as well as (PCa I + IIA) from (PCa IIB + IIC + IIIB) samples (AUC: 0.750; p = 0.023; Sn: 68.8%, Sp: 61.5%; PPV: 68.7%, NPV: 61.5%). The gene discussed is KLK3; the disease is benign prostatic hyperplasia.