We conducted further validation by analyzing the expression levels of the top transcripts (PCAT18, PCA3, and KLK3 as long RNAs, and 27b-3p, miR-30a-5p, miR-125b-5p, and miR-574-3p as miRNAs) in ddPCR using a sub-cohort of the validation sample set used for real-time PCR experiments, consisting of 40 PCa and 40 BPH samples. This evidence concerns the gene PCA3 and benign prostatic hyperplasia.