Patient 2 (Pt #2) was a 50-years-old woman diagnosed with de novo AML due to 50% of myeloid blast cells documented at diagnostic BM evaluation, expression of CD34+, CD117+, CD33+, CD13+, HLA-DR+, CD4+, and CD25+ immunophenotypic markers, NK by chromosome banding analysis, an FLT3-ITD (allelic ratio, AR = 0.8), and an NPM1 mutation (NPM1mut/ABL × 100 = 262.16 by real-time RT-PCR) at conventional molecular analysis. The gene discussed is NPM1; the disease is acute myeloid leukemia.