ANXA5 and colorectal carcinoma: Annexin-V fluorescein isothiocyanate/propidium iodide (FITC/PI) staining showed that the proportions of apoptotic cells were markedly increased in a dose-dependent manner in HCT-116 and HCT-8 CRC cells when CRC cells were incubated with concentrations of 50 and 100 μM decursin (Figure 3A,B).