Activation of Notch1 signaling is correlated with increased cell proliferation in HNSCC through its influence on cell cycle progression, while constitutive activation of NICD promotes CSC traits in differentiated HNSCC cells.Reducing Notch1 signaling attenuates CSC traits in HNSCC-derived CSCs and enhances their chemosensitivity to cisplatin by suppressing the expression of ABCC2 and ABCG2 transporter genes. This evidence concerns the gene NOTCH1 and head and neck squamous cell carcinoma.