The administration of nitroglycerin (GTN), calcitonin gene-related peptide (CGRP), and transient receptor potential cation channel subfamily A member 1 (TRPA1) agonist is the most widely used approach to induce migraine-like pain in rodents, commonly measured as mechanical allodynia in the peri-orbital area or in the paw [131,132]. Here, TRPA1 is linked to migraine disorder.