Relative WEE1 expression levels when the control (normal colon mucosa) was set as 1 was determined in seven human CRC cell lines (DLD1, KM12SM, Caco2, HT29, HCT116, LoVo, and RKO), one BALB/c mouse-derived CRC cell line (CT26), and one C57BL6 mouse-derived colon cancer cell line (MC38) using real-time PCR (Figure 2a). This evidence concerns the gene WEE1 and malignant colon neoplasm.