CDKN2A and melanoma: Nuclear expression of PRAME (Preferentially expressed Antigen in Melanoma), cyclin D1 positivity and loss of p16 on immunohistochemistry can also be helpful in distinguishing Co-M from naevi or low-grade C-MIL (both PRAME and cyclin D1 negative, p16 positive) [57,88,93,94,95,96].