The mRNA levels of SREBP1, FAS, and ACC-1 were significantly increased, while the mRNA levels of PPARα and CPT1 were significantly reduced in the livers of the T2DM + SFFE + brusatol-treated rats, as compared to the T2DM + SFFE-treated rats (Figure 5A–E). This evidence concerns the gene SREBF1 and type 2 diabetes mellitus.