Among these pathways, dif-mRNAs for the ECM were enriched mainly in ECM-receptor interaction, focal adhesion, human papillomavirus infection, the PI3K-Akt signalling pathway, amoebic disease, small cell lung cancer, actin cytoskeleton regulation, hypertrophic cardiomyopathy, and arrhythmic right ventricular cardiomyopathy and cancer (Figure 9B and Additional file 11). Here, AKT1 is linked to hypertrophic cardiomyopathy.