The KEGG pathway analysis revealed that the target genes were predominantly enriched in several signalling pathways, including the cGMP-PKG signalling pathway, the GnRH signalling pathway, the AGE-RAGE signalling pathway in diabetic complications, the AMPK signalling pathway, the TGF-β signalling pathway, the stem cell pluripotency regulation pathway, miRNAs in the cancer pathway, the lysine degradation pathway, leukocyte migration through the EC pathway, cytogenetic–cytokine receptor interactions, and glycerophospholipid metabolism (Figure 7B and Additional file 7). The gene discussed is GNRH1; the disease is cancer.