Among these pathways, dif-mRNAs for the ECM were enriched mainly in ECM-receptor interaction, focal adhesion, human papillomavirus infection, the PI3K-Akt signalling pathway, amoebic disease, small cell lung cancer, actin cytoskeleton regulation, hypertrophic cardiomyopathy, and arrhythmic right ventricular cardiomyopathy and cancer (Figure 9B and Additional file 11). This evidence concerns the gene AKT1 and human papilloma virus infection.