To investigate the molecular mechanism by which SPECC1-415aa regulates the sensitivity of GBM cells to TMZ, immunoprecipitation-mass spectrometry (IP-MS) experiments were performed in 293 T cells using a linear SPECC1-415aa overexpression plasmid with a 3 × Flag tag (p-SPECC1-415aa-Flag). This evidence concerns the gene SPECC1 and glioblastoma.