Compared with the overexpression of wild-type ADAMTS1, overexpression of proteolytic inactive ADAMTS1 led to reductions in the secretion of cleaved VCAN (Fig. 5F), EGFR activation (Fig. 5G), invasive ability (Fig. 5H), and anoikis resistance (Fig. 5I) in RCC cell lines. This evidence concerns the gene ADAMTS1 and renal cell carcinoma.