Functionally, only ZnMc treatment induced an increase in the invasive ability (Fig. 5D) and anoikis resistance (Fig. 5E) in both cell lines, suggesting that the metalloproteinase domain of ADAMTS1 is crucial for its role in mediating VCAN cleavage, EGFR activation, invasion, and anoikis resistance in RCC cells. Here, VCAN is linked to renal cell carcinoma.