Compared with Akt1s1+/+Tsc2f/f; Cre mice, Akt1s1−/−Tsc2f/f; Cre mice exhibited smaller kidneys, lower ratios of kidney weight to body weight, fewer and smaller renal cysts, and lower cyst index (Fig. 2C–F, Supplementary Fig. 2). The gene discussed is AKT1S1; the disease is cyst.