Compared with Akt1s1+/+Tsc2f/f; Cre mice, Akt1s1−/−Tsc2f/f; Cre mice exhibited smaller kidneys, lower ratios of kidney weight to body weight, fewer and smaller renal cysts, and lower cyst index (Fig. 2C–F, Supplementary Fig. 2). This evidence concerns the gene AKT1S1 and Renal cyst.