To further explore the effects of TSPAN3 on NSCLC proliferation in vivo, H1299 cells stably transfected with the TSPAN3-overexpressing plasmid or the sh-TSPAN3 short hairpin RNA (selected by G418, Supplementary Fig. 2) were subcutaneously delivered into nude mice for the assessment of tumor formation. This evidence concerns the gene TSPAN3 and non-small cell lung carcinoma.