Upon stimulation with CHX and TNF-α, a detrimental proinflammatory cytokine underlying mucosal damage in IBD, cell viability was comparable between YOD1-deficient and -sufficient Mode-K cells (Appendix Fig. S9B), decreasing the possibility that YOD1 affects colitis by regulating the apoptosis of IECs. This evidence concerns the gene TNF and colitis.