Altogether, these results indicate that mutant KIF5A overexpression does not alter basal autophagy, that in turn does not represent the preferential degradation route for WT and mutant KIF5A, despite the abnormal interaction observed between SQSTM1/p62 and the ALS-linked N999Vfs*40 KIF5A mutant. This evidence concerns the gene KIF5A and amyotrophic lateral sclerosis.