TransFac predicted activation of transcription factors (TFs) highlighted inflammatory TFs, including those belonging to the NF-κB pathway (NFKB1, RELA, NFKBIA), HDAC1, which converts fibroblast into cancer-associated fibroblasts (CAFs)46, RUNX1 which prognosticates immune infiltrate in CAFs47, as well as NEF2L2 which encodes the key antioxidant TF Nrf2, also known to promote metastasis in CAFs48 (Fig. 1N). The gene discussed is NFKB1; the disease is cancer.