FASN and cancer: Given that the qPCR results demonstrated a significant increase in FA metabolism involvement in CSCs and supporting data from the TCGA and GTEx databases also indicated a significant difference in the expression of FASN in HCC tissues and normal liver tissues (Fig. 2A), suggesting the crucial role of FASN in cancer development, we further selected FASN as the target and hypothesized that inhibiting FASN could impact CSC survival.