The aim of this study was to determine the concentrations of SEMA7A, SEMA4D, ADAM8, and ADAMTS10 proteins in CRC tissues and surgical margin tissues, and to investigate whether the KRAS, NRAS, BRAF, PIK3CA, and AKT1 gene mutations, as well as MSI status, affect the expression of these proteins. This evidence concerns the gene SEMA4D and colorectal carcinoma.