The aim of this study was to determine the concentrations of SEMA7A, SEMA4D, ADAM8, and ADAMTS10 proteins in CRC tissues and surgical margin tissues, and to investigate whether the KRAS, NRAS, BRAF, PIK3CA, and AKT1 gene mutations, as well as MSI status, affect the expression of these proteins. Here, AKT1 is linked to colorectal carcinoma.