Because previous studies from our and other groups have linked Ang II availability with both BBB dysfunction and microglia activation (Biancardi et al., 2014; Buttler et al., 2017; Mowry et al., 2021), we also analyzed the time-course changes of Ang II density within the three autonomic nuclei during the establishment of hypertension in the same ROIs of SHRs and Wistar rats. This evidence concerns the gene AGT and hypertensive disorder.