Moreover, in another neuroinflammation mouse model e.g., EAE (Experimental autoimmune encephalomyelitis), similar scenario was uncovered: as neuroinflammation is activated (Fig. 7j–l and Supplementary Fig. 6f), the level of palm-Cadm4 as well as the expression of ZDHHC3 are significantly downregulated (Fig. 7m, n), consequently, the PM localization of Cadm4 (Fig. 7o, p), the fluorescence intensity of APC and Cadm4 (Fig. 7q) are significantly impaired in EAE as compared to WT control mouse. The gene discussed is ZDHHC3; the disease is experimental autoimmune encephalomyelitis.