To verify if Cadm4 is indeed modified with protein palmitoylation, Cadm4 expressed either ectopically in N2a, a neuroblastoma cell line, HEK-293T or expressed endogenously in WT mouse brain was evaluated by Acyl-RAC assay.15,24 The results showed that Cadm4 is readily palmitoylated both in vitro and in vivo (Fig. 1a and Supplementary Fig. 1b). The gene discussed is CADM4; the disease is neuroblastoma.