MYH6 and cardiac hypertrophy: To investigate the putative role of increased Acsl4 expression in cardiac hypertrophy, we generated an Acsl4 transgenic (Acsl4 TG) mouse in which expression of the Acsl4 transgene is activated by cardiomyocyte-specific Cre (Myh6-Cre),30 thereby overexpressing Acsl4 selectively in cardiomyocytes (Fig. 2a); for these experiments, Myh6-Cre and/or loxP-stop-loxP-Acsl4 mice were used as negative controls.