Notably, immunohistochemical analysis revealed that UCP1-positive adipocytes were present throughout the development of cachexia from T-1 to T-3, prominently in the super pre-cachexia stage (T-1) in the YES2 tumour-bearing mice compared to their PF counterparts (Fig. 1k), which was supported by immunoblotting (Fig. 1l). This evidence concerns the gene UCP1 and neoplasm.