Additionally, GRP75 expression was enhanced in EVs secreted by other well-known cachexia-inducing cancer cells,17,23 including C26 (mouse colon cancer), HepG2 (human hepatocellular carcinoma), and LLC cells, relative to EVs from non-cachexia inducible cancer cell lines MC38 (mouse colon cancer) (Fig. 3d), suggesting that the upregulation of GRP75 is involved in pancancer cachexia. Here, HSPA9 is linked to malignant colon neoplasm.