Nestin-cre is expressed in some locations outside the nervous system, such as by cells in the kidney and heart [53], but these have not been reported to be sites of Sox3 expression, and while both Nestin and Sox3 are expressed quite broadly in the nervous system, the crucial regulatory role of the hypothalamus on the pituitary makes it highly likely that it is defects in this region that underlie the hypopituitarism phenotype. This evidence concerns the gene NES and hypopituitarism.