CD20+CD86+ B cells, CD20+CD27+CD10+CD24+CXCR4+cMYC+ B cells, and populations of CD20+CD27- and CD20+CXCR5- B cells are elevated in HIV+ pre-NHL (cART-naïve) compared to HIV-negative samples. Here, CXCR5 is linked to non-Hodgkin lymphoma.