CD20+CD27+CD24+CXCR4+CXCR5+ B cells, CD20+CD27+CD10+CD24+CXCR4+ cMYC+ B cells, and populations of CD20+CD27- B cells are significantly elevated in HIV+ pre-NHL (cART-naïve) compared to HIV+ cART-naïve samples. Here, CXCR5 is linked to non-Hodgkin lymphoma.