In addition, significant increases in steatosis were observed under both the EMS and LMS medium conditions compared to the NF medium for both the PNPLA3 CC wild-type and GG LAMPS (Supplementary Figure S5), demonstrating that the EMS and LMS medium formulations recapitulate lipid accumulation associated with the progression of MASLD. This evidence concerns the gene PNPLA3 and steatosis.