In addition, a significant increase in LDH secretion was observed in both NF and EMS media on day 8 and on days 4 and 6 in the LMS medium, suggesting an overall increase in cytotoxicity in the PNPLA3 GG LAMPS, consistent with its characterization as a high-risk variant associated with an increased risk for MASLD progression and liver damage (Supplementary Figure S4C) (Mann and Anstee, 2017; Mann et al., 2020; Min et al., 2014). This evidence concerns the gene PNPLA3 and metabolic dysfunction-associated steatotic liver disease.