PNPLA3 and metabolic dysfunction-associated steatotic liver disease: We examined the specific impact of the PNPLA3 rs738409 GG variant on steatosis, cytokine secretion, stellate cell activation, and fibrosis over an 8-day time course using previously published media formulated to recapitulate MASLD phenotypes (Supplementary Table S1; Supplementary Figure S1B) (Lefever et al., 2022; Saydmohammed et al., 2021).