Using a 2-stage quantitative trait loci (QTL) mapping in BXD recombinant inbred (RI) mice, Ninein (Nin) was previously identified as a candidate gene for a Chr 12 QTL (Etanq1) associated with ethanol’s anxiolytic-like properties in the light-dark box transitional model of anxiety (Putman et al., 2016). This evidence concerns the gene NIN and Anxiety.