MALAT1 and cancer: The researchers assessed the impact of this depletion on various cancer-related characteristics, including anchorage-dependent growth and cell proliferation, as determined through gene-editing assays using plastic plates and development that is anchorage-independent using flexible agar colonies tests Since epithelial cells exhibit the highest level of MALAT1 expression, the study first used modified antisense oligonucleotides (ASOs) to target lines of luminal cells, namely T47D and MCF7 cells, as shown in Fig. (1).