Although single-cell sequencing analysis did not reveal a correlation between the expression of RIG-I in CD8+ T cells infiltrating tumours and the expression of granzyme-B or IFN-γ (Fig. EV2G), in HCC and CRC tumours, infiltrating CD8+ T cells exhibited secretion of granzyme-B compared to those in peritumoural tissues (Figs. 1J and  EV2H). This evidence concerns the gene IFNG and hepatocellular carcinoma.