In conclusion, this study of mTOR inhibitors in otherwise healthy PWH on long-term, suppressive ART revealed several important significant effects of sirolimus therapy, such as decreasing peripheral blood HIV-1 DNA burden, CD4+ T cell cycling, and CD8+ T cell PD-1 expression, which suggest that immune modulatory therapies may play an important role in ongoing efforts to cure HIV infection in persons with established infection. The gene discussed is CD4; the disease is infection.