Similar to our findings with T. cruzi-infected hiPSC-CM, we detected reduced levels of ST8Sia2 and polySia in T. cruzi-infected human neuroblastoma (SH-SY5Y) cells, in addition to lower polysialylation of PSA-NCAM, expressed by this cell line (Fig 2G and 2H), or SCN5A (Fig 2E and 2H). This evidence concerns the gene NCAM1 and neuroblastoma.