As controls, the efficiency of immunostaining was demonstrated in TE671 cells (S2C Fig; lane 3), a human rhabdomyosarcoma cell previously described as positive for NCAM and PSA-NCAM [53] specificity of the antibody towards host polysialylation was demonstrated using T. cruzi trypomastigotes (S2C Fig; lane 4). The gene discussed is NCAM1; the disease is rhabdomyosarcoma.