ZFHX4 and orofacial cleft: Zfhx4/zfhx4 elimination was performed in mice and zebrafish for functional assessment because of its indispensable involvement in palatogenesis and status as a genetic contributor to orofacial clefts in humans,23 A zfhx4 splicing morpholino (MO; E2I2) was designed to bind to the border between exon 2 and intron 2 of zfhx4 to inhibit splicing in zebrafish (Figure 4A).