Based on the pivotal role of DNASE1L3 in cfDNA digestion and the generation of cfDNA with the C‐terminal end reported in the recent literature.[18, 25] To further validate the intrinsic associations between the altered cfDNA characteristics (e.g., elevated cfDNA level, decreased “C”‐starting end sequence motifs) and chemotherapy sensitivity in cervical cancer, we conducted in vitro experiment and prognosis analysis. The gene discussed is DNASE1L3; the disease is cervical cancer.