Results from MethHC dataset showed that ADCY5 was hypermethylated in 372 GBM tissues compared with normal tissues with P < 0.05 (Fig. 5A) and high expressed ADCY5 was negatively correlated with the promoter CpG methylation (Fig. 5B, P = 0.0033). This evidence concerns the gene ADCY5 and glioblastoma.