As shown in (Fig. 1A and B), ADCY1 and ADCY5 mRNA were down-regulated in GBM comparing to normal tissues with P < 0.01, while these were no significant difference in ADCY2, ADCY3, ADCY4, ADCY6, ADCY7, ADCY8, ADCY9 and ADCY10 mRNA expression. This evidence concerns the gene ADCY5 and glioblastoma.