Inflammatory responses in ALI, triggered by LPS, play a key role in causing damage to pulmonary cells and dysfunction (Li et al., 2022; Luo et al., 2021), detected by levels of inflammatory factors like TNF-α in BALF to reveal tissue lesions and lung function (Mokra and Kosutova, 2015; Tang et al., 2014; Guo et al., 2015). The gene discussed is TNF; the disease is acute respiratory distress syndrome.