Finally, to ascertain that the rescue obtained with rapamycin was related to a positive effect on autophagy, we treated zebrafish embryos with apilimod, a PIKFYVE inhibitor which was found to enhance the fusion of autophagosome and lysosome [59–61] and to improve survival of motor neurons derived from C9-ALS patients’ iPSC [62]. This evidence concerns the gene PIKFYVE and amyotrophic lateral sclerosis.