Here we show for the first time that AHCC suppresses liver fibrosis progression by induction of cytoglobin via the Toll-like receptor 2 (TLR2)-stress-activated protein kinase/Jun NH2-terminal kinase (SAPK/JNK) pathway and inhibition of collagen production via the TLR4-NF-κβ pathway in hepatic stellate cells. Here, CYGB is linked to Hepatic fibrosis.