PRF1 and neoplasm: Inflammatory bowel diseases occur because the intestinal epithelium undergoes apoptosis in response to inflammatory cytokines, not by perforin- and granzyme-mediated cytolysis.49 The increased affinity of the CD8HTM CAR allows for the maintenance and sustainability of the anti-tumor response, not solely because of the direct, synaptic interaction, but also because of the inflammatory milieu in which these T cells function.