Importantly, while normal myometrial smooth muscle tissue sections show robust expression and nuclear localization of REST, the traces of REST that persist in leiomyoma samples have a predominantly cytoplasmic localization (Fig. S2 A, B), suggesting that PRICKLE1-mediated nuclear localization of REST may be dysfunctional in uterine fibroids. This evidence concerns the gene PRICKLE1 and leiomyoma.