In a study with heterologous cell plasmid transfection, choline acetyltransferase activity, directly leading to ligand binding regulation, proves a prerequisite for α9α10 nAChR assembly and membrane stabilization, while transmembrane proteins associated with hearing loss like TMIE or TIMEM132e serve as auxiliary subunits for channel gating [52]. The gene discussed is CHAT; the disease is hearing loss disorder.