PRF1 and neoplasm: To evaluate the association between a simplified metric of tumor immune status and OS, we applied a cytolytic score used widely to measure the immune status of the tumor microenvironment (mean of log GZMA and PRF1 expression)13 to characterize tumors as cytolytic high (>median of 3.11 log2(transcripts per million (TPM)) or cytolytic low (≤median).