We examined whether the mutational status of the most frequently comutated genes in CRC (APC, PIK3CA, RNF43 and TP53), MSI status or TMB level was associated with differences in OS in patients treated with either Enco+Bini+Cetux or Enco+Cetux compared with control (Fig. 3 and Supplementary Fig. 2b (adjusted analyses)). This evidence concerns the gene RNF43 and colorectal carcinoma.