We also measured the transcription level of TNFα, the downstream gene of NF-κB, and found that the transcription level of TNFα in Ms_Rv2347c infection group was significantly increased, suggesting that Rv2347c enhanced the activity of NF-κB and promoted the transcription of downstream genes, which was consistent with the upregulation of IL-1β expression (Fig. 6C). The gene discussed is IL1B; the disease is infection.