To test whether IL-10 and/or Cxcl2 inhibit the cytotoxic activity of CD8+ T cells in our model, activated splenic CD8+ T cells were cultured with Ly6G+ cells sorted from ascites of ID8-Egfl6 and ID8 tumor-bearing mice in the presence of IL-10 or Cxcl2 NAbs. Here, CD8A is linked to neoplasm.