Therefore, we simultaneously targeted the putative PnPf2 paralogue SNOG_08565 (25.4% sequence identity) [15] and SNOG_03067 (PnEbr1), which is co-expressed with PnPf2, ToxA, Tox1 and Tox3 high during early infection (S4 Fig) and has virulence regulating orthologues (summarised in S1B Table). This evidence concerns the gene TOX3 and infection.