Our study utilized SiT, scRNA‐seq, and T‐cell receptor (TCR) analyses of tumor and LN tissues to demonstrate that the temporal heterogeneity of the spatial CD74 isoform correlates with the expanded enrichment of C1QC+ TAMs and CD8+CXCL13+ Tex cells in LN metastasis of ESCC. This evidence concerns the gene CXCL13 and esophageal squamous cell carcinoma.